Fig. 9: Non-canonical ABCB5 peptide induced an IFNγ response. | Nature Communications

Fig. 9: Non-canonical ABCB5 peptide induced an IFNγ response.

From: Integrated proteogenomic deep sequencing and analytics accurately identify non-canonical peptides in tumor immunopeptidomes

Fig. 9

a Reactivity was measured in melanoma 0D5P by the IFNγ ELISpot assay using autologous REP TILs. Representative example of three TAAs from TYR and TYRP1 and one non-canonical dORF-derived HLAIp from ABCB5 (written in red) that induced an IFNγ response. b In addition, a representative example of CD8+ T lymphocytes from PBLs is shown when re-challenged with autologous CD4+ blasts together with 1 μM of the non-canonical ABCB5 HLAIp. (No Ag: no peptide, positive control: 1x cell stimulation cocktail).c Representative images of the IFNγ ELISpot response against the non-canonical ABCB5 peptide. In a and b, T cell reactivity for every peptide was validated by ≥ 2 independent experiments. Please refer to the Methods section for boxplot parameters. Source data are provided as a Source Data file.

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