Fig. 7: Linc00899 regulates TPPP and mitotic progression in different cell lines.

a Expression of linc00899 and TPPP after RNAi-mediated depletion of linc00899 in normal retina cell line (hTert-RPE1) based on qPCR. Results are also shown for negative control siRNAs (Ctl, from Ambion) and cells treated with transfection reagent alone (Cells). n = 3 biological replicates, *P < 0.05 and ****P < 0.0001 by two-tailed Student’s t test. b Quantification of mitotic duration from time-lapse microscopy imaging after depletion of linc00899 in RPE1 cells. Number of cells analysed is n = 135 for Ctl si, n = 98 for Cells and n = 103 for linc00899 RNAi. Data are shown as median with interquartile range from two biological replicates. ****P < 0.0001 by Mann–Whitney test. c Expression of linc00899 and TPPP after RNAi-mediated depletion of linc00899 in non-tumorigenic epithelial breast cells (MCF10A). Controls are as described in a. n = 3 biological replicates, *P < 0.05 and ****P<0.0001 by two-tailed Student’s t test. d Quantification of mitosis duration from time-lapse microscopy imaging after depletion of linc00899 in MCF10A cells, as described in c. Number of cells analysed is n = 143 for Ctl si, n = 142 for Cells and n = 63 for linc00899 siRNAs. Data are shown as median with interquartile range from two biological replicates. ****P < 0.0001 by Mann–Whitney test. e Expression of linc00899 and TPPP after RNAi-mediated depletion of linc00899 in normal primary umbilical vein endothelial cells (HUVEC). Controls are as described in a. n = 3–4 biological replicates, *P < 0.05 and **P < 0.01 by two-tailed Student’s t test. f Quantification of mitotic duration from time-lapse microscopy imaging after depletion of linc00899 in HUVEC cells, as described in e. Number of cells analysed is n = 58 for Ctl si, n = 129 for Cells and n = 181 for linc00899 RNAi. Data are shown as median with interquartile range from three biological replicates. ****P < 0.0001 by Mann–Whitney test. Depletion of linc00899 in HUVEC, RPE1 and MCF10A leads to an ~8-, 12- and 16-min delay compared to cells treated with Ctl si, respectively. Data are shown as mean ± S.E.M for a, c, e. Mitotic duration in b, d, f was defined from NEBD (t = 0 min) to anaphase using bright-field microscopy. Source data are provided as a Source Data file.