Fig. 8: Gain-of-function and rescue studies of linc00899 and its effect on TPPP expression.

a Schematic diagram of ectopic overexpression of linc00899. Linc00899 and TPPP expression were analysed by qPCR after lentiviral overexpression using lincXpress vector encoding linc00899 cDNA in HeLa (left) and RPE1 cells (right). The expression was normalised to the scrambled linc00899 vector (negative control). n = 3–4 biological replicates, *P < 0.05 by two-tailed Student’s t test. b Rescue of linc00899 function after RNAi- or LNA-mediated depletion of linc00899 in HeLa cells through ectopic overexpression of linc00899. n = 3 biological replicates, *P < 0.05, **P < 0.01 and ****P < 0.0001 by two-tailed Student’s t test. c Schematic diagram of endogenous overexpression of linc00899 using CRISPRa. Linc00899 and TPPP expression were analysed by qPCR after transduction of dCas9-VP64 and gRNAs targeting different regions of the linc00899 promoter in HeLa (left) and RPE1 cells (right). The expression was normalised to the negative guide (negative control). n = 4 biological replicates, *P < 0.05, ***P < 0.001 and ****P < 0.0001 by two-tailed Student’s t test. Data are shown as mean ± S.E.M for a–c. Source data are provided as a Source Data file.