Table 2 hPGCLC medium composition.
From: A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Component | Final concentration | Supplier |
|---|---|---|
Advanced RPMI 1640 | – | GIBCO |
B27 supplement | 1% | GIBCO |
l-glutamine | 2 mM | GIBCO |
Nonessential amino acids | 0.1 mM | GIBCO |
Penicillin–streptomycin | 100 U/ml (Penicillin) | GIBCO |
|  | 0.1 mg/ml (Streptomycin) | |
BMP2 | 500 ng/ml | SCI |
Human LIF | 1 μg/ml | SCI |
SCF | 100 ng/ml | R&D systems |
EGF | 50 ng/ml | R&D systems |
ROCK inhibitor (Y-27632) | 10 μM | TOCRIS bioscience |
Poly-vinyl alcohol (PVA, 10%) | 0.25% | Sigma |
