Fig. 3: Recruitment of ERRA-FLII-LRRFIP1 complex induces UCP1 expression.

a Gene expression in brown preadipocytes treated with vehicle, FGF6, or FGF9 for 24 h. b Schematic presentation of enChIP experiment. c Relative enrichment of FLII and LRFFIP1 proteins in the dCas9-FLAG IP in cells transfected with Ucp1 gRNAs and GFP control. N = 2 per group. d Counts per million for Flii and Lrrfip1 transcripts in brown preadipocytes treated with vehicle or FGF6 for 8 h. N = 3 per group. e FLII protein level in brown preadipocytes treated with vehicle, FGF6, or FGF9 for 48 h. N = 2 per group. Thirty micrograms of total protein was used for WB. f Ucp1 expression in brown preadipocytes transfected with scrambled siRNA or siLrrfip1, followed by treatment with vehicle, FGF6, or FGF9 for 24 h. N = 3 per group. g ERRA protein level in brown preadipocytes treated with vehicle, FGF6, or FGF9 for 72 h. N = 2 per group. Thirty micrograms total protein was used for WB. h Chromatin immunoprecipitation of ERRA on the Ucp1 enhancer in brown preadipocytes treated with vehicle, FGF6, or FGF9 for 48 h. i Chromatin immunoprecipitation of ERRA on the Ucp1 enhancer in white preadipocytes treated with vehicle (DMSO) or PGE2 for 24 h. j Ucp1 expression in brown preadipocytes transfected with scrambled siRNA or siErra, followed by treatment with vehicle, FGF6, or FGF9 for 24 h. N = 3 per group. k Relative abundance of the indicated proteins detected by mass spectrometry in FLAG IP and IgG samples. N = 4–5. l Western blot for ERRA and FLII in FLAG IP and IgG. FGF6 and FGF9 were used at concentrations of 200 and 100 ng/ml, respectively. m Ucp1 expression in brown preadipocytes transfected with Erra, Flii, and Lrrfip1 cDNA. Data are presented as means ± SEM. Two-way ANOVA. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05. A representative from a total of two to three independent experiments is shown. Source data are provided as a Source Data file.