Fig. 6: RAZUL:AZUL forms an intermolecular 4-helix bundle.
From: Structure of E3 ligase E6AP with a proteasome-binding site provided by substrate receptor hRpn10
Regions displaying AZUL contacts with RAZUL α1 (a, b), the 310-helix (c), and α2 (d) showing selected interacting sidechain atoms with oxygen, nitrogen, and sulfur colored red, blue, and yellow, respectively. The structure in c is displayed as a ribbon diagram to better view the 310-helix with hydrogen bonds included as red dashed lines. The structures in a, b, and d depict helices as cylinders for simplicity. e Electrostatic surface diagram for AZUL to highlight acidic (red) and basic (blue) regions. The bound RAZUL is displayed with cylindrical helices and sticks for N-terminal acidic residues with oxygen colored red. f ITC analyses for AZUL-binding hRpn10322–366 without or with Y326 phosphorylated. g Immunoprobing of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing indicated myc-hRpn10 constructs, including with RAZUL (ΔRAZUL) or the C-terminal 13 residues deleted (1–364) or with hRpn10 Y326 substituted with phenylalanine (Y326F). Cyclophilin B (Cyp B) is used as a loading control for WCE samples. Antibodies used for immunoprobing are indicated to the left of each panel. An asterisk “*” indicates heavy chain antibody. Source data are provided as a Source Data file.
