Fig. 4: The presence of TetA induces nuoF-dependent sensitivity to aminoglycosides. | Nature Communications

Fig. 4: The presence of TetA induces nuoF-dependent sensitivity to aminoglycosides.

From: Dominant resistance and negative epistasis can limit the co-selection of de novo resistance mutations and antibiotic resistance genes

Fig. 4

a Fold tetA-induced growth reduction of the Amk4 mutant grown in media in the presence (STR16) or absence (LB) of a sub-MIC concentration of streptomycin (16 μg/ml). Error bars display the standard error of the mean (n = 8). The red dashed line shows the baseline growth without antibiotics. The asterisk indicates statistical significance compared with the baseline (Wilcoxon rank sum test, p < 0.05). b Fitness landscape of nuoF-reversed mutants competing against their ancestor at different concentrations of tetracycline and streptomycin. A more blue color, as opposed to yellow, indicates outcompetition of the nuoF mutant. c Relative growth of the tetA-carrying nuoF mutant compared with the corresponding mutant carrying an empty vector at different streptomycin concentrations and pH values. Error bars show the standard error of the mean (n = 3) and the red dashed line indicates the value of the WT to which the remaining values are normalized. Source data are provided as a Source Data file.

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