Fig. 5: Effect of different MFS efflux pumps on streptomycin sensitivity and membrane permeability in the Amk4 mutant. | Nature Communications

Fig. 5: Effect of different MFS efflux pumps on streptomycin sensitivity and membrane permeability in the Amk4 mutant.

From: Dominant resistance and negative epistasis can limit the co-selection of de novo resistance mutations and antibiotic resistance genes

Fig. 5

Various genes encoding MFS pumps were independently expressed in the E. coli nuoF + fusA mutant strain. Growth (a) and membrane permeability (b) were measured and are displayed relative to those of the same E. coli strain carrying an empty vector. Membrane permeability was measured as the increase in fluorescence resulting from Hoechst 33342 uptake. The bars show the mean of two replicates (a), and the boxplot the interquartile range between the first and third quartiles and median (internal line) with dots representing individual fluorescence measurements (n = 16) (b). The red dashed lines indicate the values of the emty expression vector to which the remaining values are normalized. Source data are provided as a Source Data file.

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