Table 1 Past introgression supported by D and f₄ statistics.

From: Stable species boundaries despite ten million years of hybridization in tropical eels

P1	P2	P3	n	C_ABBA	C_BABA	D	f₄	p
A. marmorata	A. luzonensis	A. interioris	10,290	182.7	77.1	0.406	−0.0070	0.000
A. marmorata	A. luzonensis	A. obscura	15,689	186.6	93.0	0.334	−0.0043	0.000
A. marmorata	A. bicolor	A. interioris	7772	266.3	138.4	0.316	−0.0109	0.000
A. marmorata	A. luzonensis	A. bicolor	11,542	158.1	82.8	0.313	−0.0052	0.000
A. marmorata	A. obscura	A. interioris	10,208	307.9	197.8	0.218	−0.0051	0.000
A. obscura	A. bicolor	A. interioris	8304	123.8	84.1	0.191	−0.0030	0.005
A. obscura	A. bicolor	A. marmorata	11,372	104.7	71.2	0.191	−0.0025	0.002
A. obscura	A. bicolor	A. luzonensis	12,557	113.4	80.0	0.173	−0.0022	0.008
A. marmorata	A. interioris	A. megastoma	9951	96.4	72.7	0.140	−0.0023	0.026
A. marmorata	A. luzonensis	A. megastoma	13,129	69.0	52.9	0.133	−0.0008	0.201
A. luzonensis	A. marmorata	A. bicolor	14,675	105.4	84.5	0.110	−0.0011	0.106
A. luzonensis	A. bicolor	A. interioris	14,246	228.4	191.0	0.089	−0.0015	0.062
A. luzonensis	A. interioris	A. megastoma	13,632	82.4	70.2	0.080	−0.0007	0.192
A. marmorata	A. bicolor	A. megastoma	11,134	110.9	95.0	0.077	−0.0003	0.430
A. luzonensis	A. marmorata	A. obscura	15,500	111.7	96.5	0.073	−0.0003	0.406
A. marmorata	A. obscura	A. megastoma	11,647	126.1	110.0	0.068	−0.0009	0.241
A. bicolor	A. obscura	A. marmorata	11,303	80.0	73.0	0.046	−0.0007	0.261
A. obscura	A. bicolor	A. megastoma	11,761	64.7	59.5	0.042	−0.0002	0.447
A. bicolor	A. obscura	A. luzonensis	15,856	78.1	72.1	0.040	−0.0010	0.141
A. obscura	A. interioris	A. megastoma	11,017	96.2	90.8	0.029	−0.0011	0.137
A. luzonensis	A. bicolor	A. megastoma	14,602	97.0	93.1	0.020	0.0002	0.416
A. bicolor	A. interioris	A. megastoma	10,451	84.0	82.0	0.012	−0.0007	0.213
A. luzonensis	A. obscura	A. interioris	15,143	227.1	221.7	0.012	0.0005	0.300
A. luzonensis	A. obscura	A. megastoma	15,405	107.9	106.2	0.008	−0.0001	0.461
A. obscura	A. marmorata	A. megastoma	23,165,451	596,786.0	587,910.0	0.007	—	—

Only comparisons that are compatible with the inferred phylogenetic relationships and result in positive D values are shown (for all comparisons, see Supplementary Table 9). All except the comparison in the last row are based on RAD-sequencing-derived SNP data; the last comparison is based on WGS reads of a single individual of the three species. Either A. mossambica, A. megastoma, A. interioris, or A. anguilla (in the comparison based on WGS data) were used as outgroups and the comparison resulting in the largest D value is reported when multiple of these outgroups were used. p values are based on one-sided comparisons and not adjusted for multiple comparisons. n: number of sites variable among the included species; C_ABBA: number of sites at which species P2 and P3 share the derived allele; C_BABA: number of sites at which P1 and P3 share the derived allele. Italic font is used for species names and variables.

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Table 1 Past introgression supported by D and f₄ statistics.

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