Fig. 2: Differential methylation and protein expression profiles in the cecum.
From: Impact of the gut microbiota on the m6A epitranscriptome of mouse cecum and liver
a m6A-peaks found to be differentially methylated compared to differential expression of the entire transcripts in indicated mice. Differentially methylated peaks that are also differentially expressed on transcript level are displayed in red, differentially methylated peaks that are unchanged on transcript level, in blue. m6A peaks that were not significantly changed are shown in gray. Cut-offs for differential expression are log fold change (FC) −1 to 1 and Benjamini–Hochberg-corrected p-values < 0.05. p-values were estimated from moderated t-statistics with empirical Bayes moderation using limma package, followed by Benjamini-Hochberg correction. b correlation of proteome, transcriptome and epitranscriptome of CONV and GF mice; log FC of protein levels between CONV and GF mice was compared to log FC of transcript input levels (left panel) or log FC of differentially methylated peaks (right panel) between CONV and GF mice; CONV n = 7, GF n = 6 for proteome analysis; CONV n = 15, GF n = 12 for transcriptome and differential methylation analysis. Targets significantly altered between CONV and GF on protein and transcript and m6A methylation, respectively, are displayed.
