Fig. 1: Identification of DAP as an efficient and exclusive corrector of UGA nonsense mutations.
From: 2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations
a Fractionation pathway of H7 L. inversa extract, starting from fraction F87-1. b Schematic representation of the firefly luciferase construct used to measure readthrough activity. HeLa cells were transfected with an expression vector carrying a firefly luciferase (Fluc) gene consisting of the open-reading frame encoding firefly luciferase interrupted by an intron and a nonsense codon at position 109. c Luciferase activity in HeLa cells transfected with the construct described in b carrying a UGA, UAA, or UAG PTC and incubated with DMSO, H7 extract, one of various H7 fractions, or DAP. The value of each luciferase measurement is presented in the Source Data File. d Readthrough activity on the construct described in b as a function of the G418 or DAP dose. The value of each luciferase measurement is presented in the Source Data File. All data shown in c, d are representative of two independent experiments. Source data are provided as a Source Data file.
