Fig. 3: Cellular heterogeneity within Wilms tumour organoid cultures.
From: An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
a t-SNE representation of single cells from four Wilms tumour organoid lines (51T, 80T, 88T and 101T). Cells are coloured by organoid of origin (top panel) or clustering (bottom panel). Indicated are the cell types the populations are representing based on marker gene expression (see panel b). e: epithelial, s: stromal, b: blastemal-like. b t-SNE maps showing the colour-coded logged expression levels of several markers for each population demonstrating that different cell types are present in 51T and 88T, whereas 80T and 101T organoids primarily consist of different epithelial subpopulations, which is in line with their histological appearance. c High-resolution 3D imaging of 51T Wilms tumour organoids immunolabeled for E-cadherin (E-cad; red), SIX2 (green) and CD90 (white). Bottom panels depict enlargement from top panel in 3D (left panel) and a representative optical section (others panels). Scale bars, 100 µm (top) and 50 µm (bottom). Images are representative of n = 2 independent experiments. d Immunofluorescence imaging on 51T Wilms tumour tissue sections immunolabeled for E-cadherin (E-cad; red), SIX2 (green) and CD90 (white). Scale bars 100 µm. Images are representative of n = 2 independent experiments.
