Fig. 3: Gene expression analysis of Th1 cells transduced with TAp73 or DNp73 constructs.

a–d Empty vector (Vector), TAp73, or DNp73 were expressed in Th1 cells via retroviral transduction. Transduced cells were identified as GFP⁺ and enriched by cell sorting. mRNA was isolated from GFP⁺ cells and analyzed by RNA-Seq. a Number of differentially expressed genes (fold change ≥2 and FDR < 0.05) in TAp73 versus vector and DNp73 versus vector. Upregulated genes are in red and downregulated genes are in blue. b Venn diagram showing genes differentially expressed by TAp73 or DNp73 versus vector control, with 52 genes in the intersection area, of which 37 were induced by both TAp73 and DNp73, 14 were repressed by both, and Lmo2 was inhibited by TAp73 but induced by DNp73. c Genes induced and repressed (≥2-fold difference; FDR < 0.05) in TAp73 or DNp73 transduced Th1 cells compared to vector alone. The Log₂(RPKM + 1) values were plotted and shown as scatter plots. Differentially expressed genes (≥2-fold difference; FDR < 0.05) are shown as open circles in red (higher expression) or blue (lower expression) with TAp73 (left panel) or DNp73 (right panel). d Experiments were performed as in a–c; shown are mRNA expression levels of Trp73, Ifng, Il12rb1, Il12rb2, Tbx21, and Il2ra normalized to Rpl7 in cells transduced with TAp73 or DNp73. mRNA levels were measured by qRT-PCR from three independent experiments, and representative results are shown. Source data for d are provided in a Source Data File.