Fig. 4: Discovery of p73 direct target genes.

a–e p73-specific binding sites were found via ChIP-Seq analysis from Th1 cells in which FLAG-tagged TAp73 or FLAG-tagged DNp73 was expressed. a Each column represents p73 binding in empty vector (EV), FLAG-tagged TAp73, or FLAG-tagged DNp73 within a 10-kb window [−5k, 0, +5k], centered on the p73-binding summits (indicated as position “0”). The intensity of p73-binding peaks is indicated by the intensity of cyan color, whereas “white” represents no binding. b The consensus motif for p73 was derived from the top 500 p73-binding summits using MEME. c Shown is genome-wide distribution of p73-binding sites at introns, intergenic regions (defined according to RefSeq), promoter regions (15 kb 5′ of the transcription start site), and exons/UTRs regions. d Venn diagram of genes differentially expressed upon p73 overexpression and/or directly bound by either TAp73 or DNp73. e The gene tracks represent TAp73- and DNp73-binding sites found by ChIP-Seq analysis using anti-FLAG at the Mdm2, Ifng, Il12rb2, Il2ra, and Tbx21 loci. For each peak, the P value was calculated using a dynamic Poisson distribution to capture local biases in read background. Three independent ChIP-Seq experiments were performed; representative results are shown.