Fig. 6: Trp73 gene deletion reduces disease severity in EAE mice.

a Clinical scores for groups of Trp73^−/− mice and wild-type/heterozygous (WT/Het) littermates after immunization with MOG_35–55 are shown as mean values ± S.E.M. Data were analyzed by a two-tailed unpaired t test; P values are indicated (*P < 0.05). b–e Total lymphocytes were isolated from the spinal cords from WT/Het (n = 4) or Trp73^−/− (n = 5) mice 27 days after EAE induction. b Total recovered cell numbers. c Infiltrating lymphocytes from the spinal cord were analyzed by flow cytometry and the percentage of CD4⁺ T cells is shown. d, e Total cells from the spinal cord were stimulated with MOG_35–55 for 48 h, and then the percentage of IL-17A⁺CD4⁺, FoxP3⁺CD4⁺, and IFNγ⁺CD4⁺ cells and CD25 expression (MFI) were determined by flow cytometry and shown as indicated. e IL-17A, GM-CSF, IL-6, TNF, and IFNγ levels in culture supernatant after MOG_35–55 stimulation were determined using a LEGENDplex Multi-Analyte assay. The results were normalized against total cell numbers. All data are presented as scatter dot plots with mean values indicated and analyzed by a two-tailed unpaired t test using mean ± SEM values; P values are indicated (ns P ≥ 0.05; *P < 0.05). Data are from one representative of three experiments with n = 5 mice per group in each experiment. Source data for a–e are provided in a Source Data File.