Fig. 7: Augmented IFNγ production following in vitro differentiation and enhanced pathogenic IFNγ-producing T cell induction and disease mediated by Trp73-deficient T cells in an adoptive T cell transfer model of colitis.

a–c Naive CD4⁺ cells from WT and Trp73 conditional knockout (cKO) mice were differentiated into Th1 cells for 2 days. a mRNA expression of Trp73 were assessed by qRT-PCR. Data are pooled results from two independent experiments with n = 9 mice per group in total. b Intracellular IFNγ levels were measured by flow cytometric staining; a representative FACS plot is shown on the left and summarized IFNγ expression data are shown on the right. c Secreted IFNγ levels (right panel) were measured by LEGENDplex^TM assay. Data for b, c are pooled results from three independent experiments with n = 13 mice per group in total. All data from a–c are presented as scatter dot plots with mean value indicated and P values were calculated using mean ± SEM by two-tailed unpaired Student’s t test. d–f CD4⁺CD45RB^hi T cells from WT or Trp73 cKO mice were adoptively transferred into Rag2^−/− recipients to induce colitis. Colonic histology scores at weeks 4 and 7 post-transfer are shown in d. Total colonic lamina propria mononuclear cell (LPMC) counts from the indicated time points are shown in e. f Infiltrating CD4⁺ T cells in the colon were measured by flow cytometry and the percentage and number of CD4⁺ T cells are shown. IFNγ-producing CD4⁺ T cells in the colon were determined by intracellular staining, and the percentage and number of IFNγ⁺ CD4⁺ T cells are indicated. Data for d–f are combined from two experiments with 9–10 mice per group in each experiment. In total, n = 7 mice per group were analyzed at week 4, and n = 13 (WT) and n = 12 (Trp73 cKO) mice per group were analyzed at week 7. All data from d–f are presented as scatter dot plots with mean ± SD indicated and analyzed by two-tailed unpaired Student’s t test with Welch’s correction, and the P values are indicated (ns P ≥ 0.05; *P < 0.05; **P < 0.01). Source data for a–f are provided in Source Data Files.