Table 2 Summary of binding lifetimes of UvrA-YPet and Mfd-YPet after UV exposure.

From: Single-molecule live-cell imaging visualizes parallel pathways of prokaryotic nucleotide excision repair

Strain (genotype)

Probe

Treatment

Afast (%)

Ï„fast (s)

Aslow (%)

Ï„slow (s)

kb (s−1)

Exponential fit

Figure

uvrA-YPet Δmfd

UvrA-YPet (chr)

20 Jm−2 UVC (temporally averaged)

77 ± 3

1.6 ± 0.1

23 ± 3

13.1 ± 0.6

6.2 ± 0.2

2

5

uvrA-YPet Δmfd

UvrA-YPet (chr)

20 Jm−2 UVC (0–25 min)

66 ± 1

1.6 ± 0.3

34 ± 1

9.6 ± 1

5.2 ± 0.3

2

5

uvrA-YPet Δmfd

UvrA-YPet (chr)

20 Jm−2 UVC (25–50 min)

80 ± 5

1.3 ± 0.1

20 ± 5

13 ± 1

5.6 ± 0.4

2

5

uvrA-YPet Δmfd

UvrA-YPet (chr)

20 Jm−2 UVC (50–75 min)

76 ± 5

1.9 ± 0.3

24 ± 5

15 ± 2

6.7 ± 0.3

2

5

uvrA-YPet Δmfd

UvrA-YPet (chr)

20 Jm−2 UVC (75–100 min)

83 ± 3

1.5 ± 0.3

17 ± 3

15 ± 4

6.5 ± 0.5

2

5

uvrA-YPet

UvrA-YPet (chr)

20 Jm−2 UVC (temporally averaged)

74 ± 2

1.5 ± 0.1

26 ± 2

9.9 ± 0.4

5.5 ± 0.1

2

5

uvrA-YPet

UvrA-YPet (chr)

20 Jm−2 UVC (0–25 min)

71 ± 0.1

2.6 ± 0.7

29 ± 0.1

12 ± 3

4.8 ± 0.1

2

5

uvrA-YPet

UvrA-YPet (chr)

20 Jm−2 UVC (25–50 min)

70 ± 3

2.2 ± 0.4

30 ± 3

11.6 ± 0.9

5.9 ± 0.3

2

5

uvrA-YPet

UvrA-YPet (chr)

20 Jm−2 UVC (50–75 min)

78 ± 2

1.4 ± 0.1

22 ± 2

10.2 ± 0.6

5.6 ± 0.2

2

5

uvrA-YPet

UvrA-YPet (chr)

20 Jm−2 UVC (75–100 min)

87 ± 2

1.2 ± 0.2

13 ± 2

8.4 ± 0.6

5.8 ± 0.4

2

5

mfd-YPet

Mfd-YPet (chr)

20 Jm−2 UVC (temporally averaged)

  

100

12.0 ± 0.6

6.0 ± 0.1

1

5

mfd-YPet

Mfd-YPet (chr)

20 Jm−2 UVC (0–25 min)

  

100

13.7 ± 0.8

5.2 ± 0.2

1

5

mfd-YPet

Mfd-YPet (chr)

20 Jm−2 UVC (25–50 min)

  

100

11.5 ± 0.8

6.0 ± 0.2

1

5

mfd-YPet

Mfd-YPet (chr)

20 Jm−2 UVC (50–75 min)

  

100

10 ± 1

6.9 ± 0.2

1

5

mfd-YPet

Mfd-YPet (chr)

20 Jm−2 UVC (75–100 min)

  

100

8 ± 1

6.4 ± 0.3

1

5

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