Fig. 5: SSRP1 N-Terminal domain dependent stimulation of DNA replication through histone H1 chromatin eviction.

a Representative autoradiography of a DNA replication assay showing α-³²P-dCTP incorporation for 120 min in SN pre-incubated with 200 ng/μl recombinant SSRP1 or equimolar amounts of its mutated versions, as indicated. b Graph indicating fold change in somatic DNA replication efficiency relative to buffer considered as 1. SN were pre-incubated with 200 ng/μl recombinant SSRP1 or equimolar amounts of its mutated versions as indicated. Bars represent mean ± SEM. n = 3 independent experiments; p < 0.005 when comparing all values. Two-way anova. c WB of chromatin isolated at 60 min after addition to interphase egg extracts of SN pre-incubated with 200 ng/μl recombinant SSRP1 or equimolar amounts of its mutant versions and probed with the indicated antibodies. d WB of chromatin isolated at the indicate times from interphase egg extracts supplemented with SN pre-incubated with Flag-∆NTD or control buffer. e WB of chromatin isolated from interphase egg extracts incubated for 60 min with SN exposed to Flag-NTD or control buffer in the presence or absence of purified histone H1. f WB of chromatin isolated at the indicate times from interphase egg extracts incubated with somatic nuclei exposed to Flag-NTD or control buffer.