Fig. 7: SSRP1 induced development acceleration through cell cycle shortening.

a Representative images of control buffer (CTRL) and Myc-SSRP1 (SSRP1) injected Xenopus laevis embryos at 24 and 48 hours pf. Size bar = 1000 µm. b Graph showing lengths of CTRL and SSRP1- injected embryos measured 24 and 48 h pf. Each point represents one embryo. Bars show mean ± SEM; ****p < 0.0001; ns = not significant; n = 120 embryos; unpaired two-tailed t test. c Graph showing average length of cell cycle post-MBT per embryo measured for 30 cells starting at the 12th cell division using four embryos for each indicated condition. Cell cycle duration times between two sequential divisions were manually annotated from movies; n = 120 cell cycles; *p < 0.01; unpaired two-tailed t-test. d Representative images of CTRL and Myc-∆NTD—injected embryos at 24 and 48 h post-fertilization. Size bar = 1000 µm. e Graph showing length of CTRL and Myc-∆NTD mRNA injected embryos measured 24 and 48 h pf. Each point represents one animal. Bars show mean ± SEM; n = 100 embryos; **p < 0.01; unpaired two-tailed t test. f Graph showing average duration of cell cycle post-MBT measured as in (c); n = 120 cells; *p < 0.01; unpaired two-tailed t test. g Representative images of CTRL and NTD- injected embryos 24 and 48 h pf. All images are at identical scale. Size bar = 1000 μm. h Graph showing length of CTRL and NTD mRNA injected embryos measured 24 and 48 h pf. Each point represents one embryo. Bars show mean ± SEM; n = 70 embryos; ****p < 0.0001; unpaired two-tailed t test. i Graph showing average duration of cell cycle post-MBT measured as in (c); n = 120 cell cycles; *p < 0.01; unpaired two-tailed t test. For graphs in (c), (f), (i), box height represents the 25th to 75th percentiles and the centerline represents the median. The whiskers extend to the farthest data point.