Fig 1: Supplemented PUFAs are robustly incorporated into membrane phospholipids in vitro and in vivo. | Nature Communications

Fig 1: Supplemented PUFAs are robustly incorporated into membrane phospholipids in vitro and in vivo.

From: Lipidomic and biophysical homeostasis of mammalian membranes counteracts dietary lipid perturbations to maintain cellular fitness

Fig 1

Supplementation of culture media with PUFAs for 3 days (20 μM) leads to a dramatic increase in (a) ω−3 PUFA-containing membrane lipids in RBL cells supplemented with DHA and (b) ω−6 PUFA-containing membrane lipids supplemented with AA. (c) Both treatments result in significantly increased overall unsaturation of membrane lipids. The unsaturation index reflects a concentration-weighted average lipid unsaturation. (d) Mice fed a diet rich in fish oil (FO) have significantly more lipids containing ω−3 PUFAs compared to corn oil (CO) fed mice. (e) Incorporation of dietary ω−3 PUFAs increases cardiac tissue lipids containing very highly unsaturated (5 or 6 double bonds) acyl chains, resulting in an (f) increase in the overall unsaturation of membrane glycerophospholipids. Individual experiments (a–c) or animals (d–f) are shown. Bars represent mean ± SD. **p < 0.01, ***p < 0.001 for unpaired t test compared to untreated. Treatment with saturated (PA) or monounsaturated (OA) fatty acids in these conditions had no effect on the lipidome (see Fig. S2). Source data are provided as a Source Data file.

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