Fig. 3: Effects of polymorphisms on targets. | Nature Communications

Fig. 3: Effects of polymorphisms on targets.

From: Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes

Fig. 3

Percent of genes containing at least one good target (blue) and percent of good targets out of possible targets as a function of DRA frequency threshold (orange). This analysis is based on N = 654 An. gambiae samples (Ag1000G data). DRA frequency threshold is the value beyond which alternative alleles are considered to be DRAs and are filtered out (i.e., a DRA frequency threshold of 0.01 means, alternative alleles with a frequency below 1% are ignored during filtering). Note the constant decline in the fraction of good targets (orange), which is not mirrored in the fraction of genes containing good targets (blue) until the DRA frequency threshold is set at <0.01. Source data are provided as a Source Data file.

Back to article page