Fig. 3: RiboTag IP/qPCR confirms the presences of a subset of transcripts whose translation is upregulated and downregulated in oocytes depleted of DAZL. | Nature Communications

Fig. 3: RiboTag IP/qPCR confirms the presences of a subset of transcripts whose translation is upregulated and downregulated in oocytes depleted of DAZL.

From: The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation

Fig. 3

Representative mRNA targets affected by DAZL removal. RiboTag/RNA-Seq data are reported in a down and b up. Ribosome loading DAZL-MO/CON-MO for the same transcripts assessed in independent biological replicates by RiboTag IP/qPCR is reported in c, down and d up. Data are reported as ratio DAZL KD/control. Dppa3 and CcnB1 are used as negative control in a, c. Gdf9 mRNA is used as negative control in b, d. Experimental conditions are identical to those described in Fig. 2b except for the qPCR analysis. Bars are the average of two or more observations. P values in panel C were calculated with the Bonferroni-Dunn method, with alpha = 0.05. Each gene was analyzed individually, without assuming a consistent SD. P values are the following: Dppa3 = 0.851918, Dazl = 0.015042, Tex19.1 = 0.035962, Btg4 = 0.069233, Rad51C = 0.059755, Ireb2 = 0.006635, Tcl1 = 0.032710, CcnB1 = 0.327854.

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