Fig. 3: Crystal structure of the NELL2–Robo3 complex.
From: NELL2-Robo3 complex structure reveals mechanisms of receptor activation for axon guidance
a The structure of hRobo3 (green) bound to hNELL2 (cyan). The three calcium ions are depicted as balls; the two glycan moieties and the side chains they are linked to are shown as sticks. b Interactions of the EGF2 domain with Robo3 are dominated by polar contacts. c Interactions of the EGF3 domain with Robo3 are exclusively hydrophobic. d Binding isotherms for biotinylated wild type and mutant hNELL2 EGF1–6 measured against S2 cells expressing hRobo3 FN1–3. y-axis represents mean fluorescence intensity (MFI) from fluorescent streptavidin. e Binding isotherms for biotinylated wild type and mutant hRobo3 FN1–3 measured against S2 cells expressing hNELL2 EGF1–6. Apparent KD values for S2 cell staining experiments are listed in Supplementary Fig. 3a.
