Fig. 4: Interplay between Ubx and the lineage-restricted TFs Tin and Grh.

a Left panel: Venn diagram representing the overlap of genomic regions (1 kb) bound by Ubx (mesoderm ChIP-seq²) and Tin (ChIP-on-ChIP⁴⁵). 251 regions are co-bound by Ubx and Tin in the mesoderm, which are located in the vicinity of 367 genes. Fold enrichment of gene ontology terms of genes commonly bound by Ubx and Tin in the mesoderm. Right panel: Venn diagram representing the overlap of genomic regions (1 kb) bound by Ubx (neural ChIP-seq²) and Grh (ChIP-seq⁴⁶). 401 regions are co-bound by Ubx and Grh in the nervous system, which are located in the vicinity 604 genes. Fold enrichment of gene ontology terms of genes commonly bound by Ubx and Grh in the nervous system. b Graphical representation in percentage of the genomic localisation of region bound in common by Ubx-Tin and Ubx-Grh classified into promoter regions (−2 kb from TSS), gene bodies (5′-UTR, exon, intron, 3′UTR), and intergenic regions. c Venn diagram representing the overlap of genomic regions (1 kb), which are bound by Ubx-Tin and Ubx-Grh. Only 38 regions are bound by both TF combinations revealing a distinct signature of Ubx-Tin (85% exclusive) and Ubx-Grh (90% exclusive) bound cis-regulatory elements. d Top panel: Venn diagram representing the overlap of genes bound by Ubx and Tin in the mesoderm and by Ubx and Grh in the nervous system. Bottom panel: Representative motifs identified by AME motif search (MEME suite) within 1 kb region co-bound by Ubx and Tin in the mesoderm and Ubx and Grh in the nervous system. e Venn diagrams representing the overlap of genes bound by Ubx and Tin with the mesodermal transcriptome (mesodermal RNA-seq²) (left panel) and genes bound by Ubx and Grh with the neural transcriptome (neural RNA-seq²) (right panel). GO term and motif p-value are calculated with Fisher test. See also Supplementary Table 2 and Source Data file. Source data are provided as a Source Data file.