Fig. 2: Single-channel recordings of N2A- and N2B-containing NMDARs under continuous DNRAb exposure. | Nature Communications

Fig. 2: Single-channel recordings of N2A- and N2B-containing NMDARs under continuous DNRAb exposure.

From: Lupus autoantibodies act as positive allosteric modulators at GluN2A-containing NMDA receptors and impair spatial memory

Fig. 2

a, b Example single-channel recordings of hGluN1/hGluN2A (a) and hGluN1/hGluN2B (b) with B1 (left traces) or G11 (right traces) antibodies at 10 μg/ml. Recordings were made in the on-cell configuration (holding potential, +100 mV). Downward deflections are inward currents. Low resolution top traces show 12 s of recording (filtered at 1 kHz); more high-resolution bottom traces (selection in black box) show 230 ms (filtered at 3 kHz). c Equilibrium open probability (Eq. Popen) (mean ± SEM) for N2A- or N2B-containing NMDARs (from left to right for hN1/nN2A, n = 6, 7, 8, 8 cells recorded; for hN1/hN2B, n = 6, 6, 6, 10 cells recorded). Antibody was either B1 (gray) or G11 (green) (*p < 0.05, two-sided t test) (left to right for N2A, p = 0.0335, 0.0226; left to right for N2B, p = 0.745, 0.0556). d DNRAbs enhance forward rates of activation. Frequency of single-channel openings (mean ± SEM) derived from samples in c with antibody either B1 (gray) or G11 (green) (*p < 0.05, one-sided t test) (left to right, p = 0.0270, 0.212). Test DNRAbs was 100 μg/ml.

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