Fig. 2: Crystal structure of apo NtMOC1.

a Schematic domain structure of NtMOC1. The C-terminus (residues 108-275) is the RuvC-like domain, the structure of which was determined in this study. b Two perpendicular views of the overall structure of apo NtMOC1. Secondary structural elements are labeled in protomer B. Dashed lines indicate residues with missing electron density. c Topological diagram of the NtMOC1 protomer. Secondary structure elements are labeled as in a. Two β-sheets (β4–β5) observed in the complex structure are shaded in gray. The electron density of this segment is missing in the apo structure. d Residues with small side chains constitute the dimer interface. e Dimer interface of the helix bundle. α2s from protomers A and B are shown in the electrostatic surface potential and in the yellow cartoon illustration, respectively. The blue and red colors indicate positively and negatively charged residues, and white regions indicate hydrophobic residues.