Fig. 2: Guanylate cyclase acts in olfactory neurons to regulate fat metabolism. | Nature Communications

Fig. 2: Guanylate cyclase acts in olfactory neurons to regulate fat metabolism.

From: Olfactory specificity regulates lipid metabolism through neuroendocrine signaling in Caenorhabditis elegans

Fig. 2

a The [daf-11::GFP] integrated transgenic array shows the expression of daf-11 in seven pairs of head neurons: ASK, ADL, ASI, AWB, ASH, ASJ, and AWC. Scale bar = 10 μm. b Mosaic analysis using the [daf-11::RFP] extrachromosomal transgenic array discovers that daf-11 expression in AWC olfactory neurons sufficiently rescues the increased fat storage in the daf-11 mutants. Individual worms that are mosaic for the transgenic array expression, are numbered 1–12 in the bar chart and in the table presenting the expression pattern. Error bars represent s.d. Individual worm images are in Supplementary Fig. 1b. c Using the ceh-36 promoter, specific restoration of daf-11 only in AWC neurons is sufficient to suppress the increased fat storage in the daf-11 mutants. Representative SRS images are in Supplementary Fig. 6a. d Using the ssu-1 promoter, restoration of daf-11 expression specifically in ASJ neurons does not suppress the fat storage increase in the daf-11 mutants. Representative SRS images are in Supplementary Fig. 6b. For c and d, data are mean ± s.d., ***P < 0.001, n.s. not significant by two-way ANOVA with Sidak’s multiple comparison test. For c–d, numbers of animals used are listed in Supplementary Data 1. For b–d, source data are provided as a Source Data file.

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