Fig. 4: Specific FOXO isoforms mediate the olfactory regulation of fat metabolism.
a Inactivation of the nuclear hormone receptor DAF-12 does not suppress the increased fat storage in the daf-11 mutants with only AWCOFF neurons. Representative SRS images are in Supplementary Fig. 6i. b The daf-2 mutation leads to fat storage increase, and further enhances the fat storage increase in the daf-11 mutants. Representative SRS images are in Supplementary Fig. 6j. c DAF-16 is required for the increased fat storage in the daf-11 and nsy-5 mutants, that lack AWCON neurons, and in the glc-3 mutants with activated AIY neurons. Representative SRS images are in Supplementary Fig. 7a. d The daf-16 deletion fully suppresses the increased fat storage in the daf-11 mutants, which can be rescued by restoration of the daf-16d/f, but not the daf-16a or daf-16b isoform. Intestine-only but not neuron-only restoration of the daf-16d/f isoform sufficiently rescues the fat storage increase in the daf-11; daf-16 double mutants. Representative SRS images are in Supplementary Fig. 7b. For a, b, c data are mean ± s.d., **P < 0.01, ***P < 0.001, n.s. not significant by two-way ANOVA with Sidak’s multiple comparison test. For d, data are mean ± s.d., ***P < 0.001, n.s. not significant by one-way ANOVA with Tukey’s multiple comparison test. Numbers of animals used are listed in Supplementary Data 1. For a–d source data are provided as a Source Data file.
