Fig. 5: SGK-1 kinase regulates fat metabolism in response to olfactory signaling.
a Neither DAF-16A nor DAF-16D/F nuclear localization is strongly enhanced in the daf-11 mutants, as it does in the daf-2 mutants. Scale bar = 40 μm, yellow dashed lines circle nuclei of the intestinal cells. b Inactivation of SGK-1 fully suppresses the increased fat storage in the daf-11 mutants. Intestine-specific restoration of sgk-1 rescues the fat storage increase in the daf-11; sgk-1 double mutants. Representative SRS images are in Supplementary Fig. 7c. c, d Neither the JNK homolog jnk-1 (c), nor the AMPK homolog aak-2 (d) affects the fat storage level in WT or in the daf-11 mutants. Representative SRS images are in Supplementary Fig. 7d, e. e Knockdown of sgk-1 in the daf-11 and nsy-5 mutants, that lack AWCON neurons, or in the glc-3 mutants with active AIY neurons suppresses the increased fat storage. Representative SRS images are in Supplementary Fig. 7a. For b–e data are mean ± s.d., ***P < 0.001, n.s. not significant by two-way ANOVA with Sidak’s multiple comparison test. Numbers of animals used are listed in Supplementary Data 1. For b–e source data are provided as a Source Data file.
