Fig. 1: An inhibitor of the transcriptional activity of TEAD triggers rapid keratinocyte cell cycle arrest and differentiation. | Nature Communications

Fig. 1: An inhibitor of the transcriptional activity of TEAD triggers rapid keratinocyte cell cycle arrest and differentiation.

From: YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity

Fig. 1

a Schematic representation of TEADi and IF showing its nuclear localization in HEK293 cells. b Transcriptional activity of TEAD measured by luciferase assay with a reporter containing tandem TEAD-binding sites in HEK293 cells. c Coimmunoprecipitation experiments in HEK293 cells to show TEADi reduces TEAD interaction with YAP1 and TAZ. d Luminescence cell proliferation assay in N/TERT2G cells transduced with adenoviruses expressing GFP (Ad-GFP, control) or TEADi (Ad-TEADi). e qRT–PCR analysis of expression of indicated markers in N/TERT2G keratinocytes transduced with GFP (Ad-GFP, control) or TEADi (Ad-TEADi) for 48 h. Values are indicated in fold over GFP control. f Western blot analysis of expression of basal and differentiation markers in N/TERT2G cells transduced with GFP (Ad-GFP, control) or TEADi (Ad-TEADi) for 48 h. g Epidermis reconstruction assay with N/TERT2G keratinocytes stably expressing a tetracycline-inducible GFP (control) or TEADi. Arrows indicate aberrant differentiation. h IF staining showing the expression of proliferation and differentiation markers in the organotypic epidermis. In b, mock n = 6, YAP1 n = 3, TAZ = 3 biological replicates each; in d and e n = 3 biological replicates. Mean ± SD is shown; in b and e two-tailed unpaired t test used and in d two-way ANOVA with Sidak’s multiple comparison test. Source data are provided as a Source Data file.

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