Fig. 3: Regulation of keratinocyte differentiation by TEAD and KLF4.

a Selected factors found in the transcription factor binding site enrichment analysis of TEADi upregulated and downregulated gene sets. b IF staining and quantification showing the expression of KLF4 in N/TERT2G cells following transduction with GFP (Ad-GFP, control) or TEADi (Ad-TEADi) for 48 h. c Western blot analysis of expression of indicated markers in N/TERT2G cells transduced with pooled siRNAs for KLF4 for 24 h and GFP (Ad-GFP, control) or TEADi (Ad-TEADi) for additional 48 h. d Coimmunoprecipitation experiments in N/TERT2G keratinocytes transduced with pooled siRNAs for KLF4 for 48 h. e Western blot analysis in N/TERT2G keratinocytes transduced with lentiviruses expressing GFP or KLF4 for 48 h. f Transcriptional activity of TEAD measured by luciferase assay with a reporter containing tandem TEAD-binding sites in HEK293 cells transduced with pooled siRNAs for KLF4 for 48 h. g Graph indicating the GO biological process terms enriched in upregulated (q < 0.05, FC ≥ 1.5) and downregulated (q < 0.05, FC ≤ −1.5) genes by KLF4 knockdown (siKLF4) in N/TERT2G keratinocytes. h Selected upstream transcriptional regulators for genes in g generated using IPA. i Venn diagrams showing the gene number overlap of differentially regulated genes (q < 0.05, |FC| ≥ 1.5) between the indicated datasets in N/TERT2G keratinocytes. j Graph indicating the fold change (Log2 FC) of genes related to keratinocyte differentiation in the indicated datasets. Only differentiation genes differentially regulated in the TEADi 48 h dataset are shown. k Western blot analysis in N/TERT2G keratinocytes treated with or without siYAP1/TAZ and then transduced with adenoviruses expressing GFP or TEADi. In b n = 27 fields from 3 biological replicates, (f) n = 3 biological replicates. Mean ± SD is shown; two-tailed unpaired t test. In (i) p indicates the p-value of the overlap in Venn diagrams, Fisher’s exact test. Source data are provided as a Source Data file.