Fig. 3: Substrate scope of BrtB and nature of the nucleophile. | Nature Communications

Fig. 3: Substrate scope of BrtB and nature of the nucleophile.

From: BrtB is an O-alkylating enzyme that generates fatty acid-bartoloside esters

Fig. 3

a Mono- and diesters are formed upon incubation of BrtB and 1 with different fatty acid substrates for 10 h. Conversion values were obtained from areas of reaction product EIC peaks obtained using LC-HRESIMS analysis and assuming similar ionization behavior. Full reactions were carried out in duplicate and contained 0.8 μM recombinant NStrep-BrtB, 100 μM of 1 and 200 μM of fatty acid in reaction buffer. b LC-HRESIMS analysis of BrtB esterification reactions between 1 and [18O2]acetic acid or non-labeled acetic acid indicate that two 18O atoms are incorporated into the product per ester moiety.

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