Fig. 4: Evaluation of the devised “pocket-escaping” probe for in vivo imaging of liver fibrosis in mice.
a The illustration of fibrosis mediated by allysine. b The mechanism of developing probe for allysine. c, d Fluorescence photograph and quantification analysis of BNLBN and NLBN (10 µM for each) in imaging O-SA (0−10 µM). e Molecular logic gates for illustrating the backdoor problem of NLBN on the detection. f In vivo tracing liver fibrosis by BNLBN and NLBN (100 μL, 200 μM, intravenously for each of these two probes). g Tissue fluorescence images with BNLBN and NLBN in liver, lung, heart, spleen, and kidney. h Fluorescence imaging of liver in different model by BNLBN. i Masson staining of liver tissues from mice. Scale bar = 100 µm. j LOX activity assay of CCl4-induced liver fibrosis model. Data are represented as mean values ± SD (n = 5 mice for each group). Statistical significance was determined by one-way ANOVA with Tukey’s test. p = 0.0152 for 2-w CCl4 treated group versus the control group. p < 0.0001 for 4-w CCl4 treated group versus the control group. blank: without any treatment; control: treated with PBS; mild fibrosis: twice a week, intraperitoneal injection of CCl4 (10% in olive oil; 1 mL/kg, for 2 weeks); severe fibrosis: the same treatment as but for 4 weeks. Source data for Fig. 4j are provided as a Source Data file.
