Fig. 2: hPSC-RPE induction in 2D using hrLN-111 and hrLN-521. | Nature Communications

Fig. 2: hPSC-RPE induction in 2D using hrLN-111 and hrLN-521.

From: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells

Fig. 2

a Camera pictures showing the progression of the differentiation (note pigmentation, weeks 3–7) in suspension cultures (embryonic bodies, EB) and 2D in both hrLN-111 and hrLN-521. b Bright field and immunofluorescence pictures showing the increase of CD140b expression and pigmentation level during the time course of 2D hPSC-RPE differentiation on hrLN-521 and hrLN-111. c Charts comparing the yield and percentage of positive cells, measured by flow cytometry, for CD140b and TRA-1-60 during the time course of hPSC-RPE differentiation among the three different protocols tested (embryoid bodies, hrLN-521 and hrLN-111). d Gene expression analysis of pluripotency and RPE-specific genes throughout the time course showed in a. Values are normalized to GAPDH and displayed as relative to undifferentiated hESC. Bars represent means ± SEM from three independent experiments. Scale bars: a = 5 mm; b = 100 μm. Source data are provided as a Source Data file.

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