Fig. 5: CD56 as a potential surface marker for immature hPSC-RPE. | Nature Communications

Fig. 5: CD56 as a potential surface marker for immature hPSC-RPE.

From: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells

Fig. 5

a Box plot comparing NCAM1/CD56 median expression between the RPE and eye-field clusters measured by the 10x Genomics single-cell RNA-sequencing experiment described in Fig. 4. Eye-field cluster: Median = 0.36, minimum =  0.0, maximum = 1.66, interquartile range = 0.58; RPE cluster: Median = 0.0, minimum = 0.0, maximum = 1.38, interquartile range = 0.0. b Bright field and immunofluorescent images showing the co-expression of the retinal progenitor marker PAX6 together with extracellular markers CD140b and CD56 in hPSC-RPE after 60 days of differentiation. c Flow cytometry dot plots comparing percentage of cells expressing high levels of CD56 cell surface protein at day 60 of differentiation in cells that were either replated or enriched for CD140b+/CD184− or CD140b+/GD2− at the 30-day replating step. d Upper: Bright field and immunofluorescence pictures displaying the absence of CD56 (NCAM1) expression in albino rabbit retinas injected with pigmented hPSC-RPE cells. Lower: Bright field immunohistochemistry pictures showing the absence of CD56 (NCAM1) in a human subretinal tissue section. Subretinal hPSC-RPE cells in albino rabbit were stained for human-specific NuMa antigen to distinguish them from native RPE cells. Scale bars: b = 100 μm; d = 50 μm.

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