Fig. 7: Adamts18 impinges on transcription and regulates cell signaling.
From: The secreted protease Adamts18 links hormone action to activation of the mammary stem cell niche
a Volcano plot showing genes, which are differentially expressed between contralateral glands transplanted with Adamts18−/− and WT epithelia; n = 3, Kolmogorov–Smirnov test, all highlighted genes have p-values < 0.05. Genes with log2(FC) >0.5 in red and log2FC <0.5 in blue. Names of selected genes are indicated. b Enrichment map plot of Reactome pathway analysis (ReactomePA) on genes downregulated in 3 pairs of contralateral glands engrafted with WT and Adamts18−/− epithelia in 3 independent experiments with 3 different donors. c CNE plot of ReactomePA of genes down regulated in contralateral glands transplanted with WT and Adamts18−/− epithelia. d Bar graphs showing relative transcript levels of Adamts18, Itga3, Itgb4, and Itgbt, normalized to Hprt in 5 pubertal host mice bearing contralateral transplants of WT and Adamts18−/− epithelia. Data represent mean ± SD. Unpaired Student t-test, two-tailed. e Bar graphs showing relative transcript levels of Fgfr2, Ctgf, and Gata3 normalized to Hprt in contralateral glands transplanted with WT and Adamts18−/− epithelia, n = 6. f Representative IF for Sma (green) and YAP (red) counterstained with DAPI (blue) of 4th mammary gland sections from 5-week-old WT and Adamts18−/− littermates; n = 3. Arrows indicate YAP positive nuclei of myoepithelial cells. g Dot plot showing quantification of relative mean intensity of nuclear YAP detected in myoepithelial cells of 5-week-old WT and Adamts18−/− littermates; n = 3. Each point represents an individual TEB. h Bar graphs showing relative transcript levels of Adamts18, Col18a1, Cited-1, Ctgf, Fgfr2, Gata-3, Itga3, and Itgb4, normalized to Hprt in pubertal WT, Col18a1−/−, Adamts18−/−, and DKO; n = 9, 8, 4, and 4, respectively. Data represent mean ± SD, one-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, n.s. not significant.
