Fig. 1: Cep55-knockout mice are viable.

a Schematic representation of mouse Cep55 protein domains (EABR, ESCRTs and ALIX-binding domain; MB, midbody) and Cep55 genomic locus, showing wild-type allele (+), the knockout first allele tm1a (including the selection cassette (neo), the LacZ trapping cassette, and LoxP and FRT recombination sites), the conditional allele tm1c (F, floxed), and the deletion allele tm1d (−). b PCR analysis of primary mouse tail tip fibroblasts (TTFs) with primers P1–4 shown in a to verify Cep55 status; n = 3 independent experiments. c Images of newborn (P0) mice of the indicated genotypes. Dotted lines indicate skull shape. d Body weights of mice of the indicated genotypes and developmental stages. Horizontal bars indicate mean; n = 5, 20, 5; 17, 24, and 14 mice, respectively; P-values calculated using one-way ANOVA followed by Dunnett’s multiple comparisons test. e Western blott of protein extracts from TTFs and mouse embryonic fibroblasts (MEFs) of the indicated genotypes with antibodies against Cep55 and Gapdh; n = 3 independent experiments. Cep55^−/− in b, c, d, and e indicates Cep55^tm1a/tm1a mice. Source data for e and d are provided as a Source Data file.