Fig. 7: ESCRTs are required for abscission in neural progenitors but not in primary fibroblasts. | Nature Communications

Fig. 7: ESCRTs are required for abscission in neural progenitors but not in primary fibroblasts.

From: Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions

Fig. 7

a Scheme of Chmp4B knockdown in Chmp4C−/− fibroblasts and wild-type NPCs. siRNA, small interfering RNA. b Chmp4C−/− fibroblasts were transfected with the indicated siRNAs for 60 h as in a and extracts analyzed for Chmp4B and α-tubulin or actin. CTR, control; si, small interfering RNA; n = 1 and 2 independent experiments, respectively. c Time-lapse images of Chmp4C−/− fibroblasts depleted of Chmp4B as shown in a. Arrowheads indicate the intercellular bridge. d Quantification of abscission success or failure in dividing fibroblasts, as defined in c. n = 3 mice per condition; 117, 127, 128, 125, 163, and 115 cells quantified, respectively. P-values were calculated using two-way ANOVA, each mean compared with Chmp4C+/+ on plastic, followed by Dunnett’s multiple comparisons test. e Time-lapse images of wild-type NPCs depleted of Chmp4B as shown in a. f Quantification of abscission success or failure in dividing NPCs, as defined in e. n = 3 mice per condition; 62 and 45 cells quantified respectively. P-values calculated using Student’s two-tailed unpaired t-test. g Model of the different abscission requirements in vivo. Red crosses indicate dying cells. All bar charts show mean ± SD. Source data for b, d, and f are provided as a Source Data file.

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