Fig. 10: CD68 promoter driven selection of MC for differentiation.
From: Endothelialization of arterial vascular grafts by circulating monocytes
a Schematic of lentiviral vectors encoding for ZsGreen and puromycin phosphotransferase under the CD68 promoter (CD68-Pr; pCD68-ZsG-Puro) and Schematic of lentiviral dual promoter (LVDP) encoding for ZsGreen under the CD68-Pr and DsRed under the human (h)PGK promoter (pCD68-LVDP). The two transcriptional units are separated by polyadenylation (SPA, synthetic polyA), terminator (Tactb), and insulator (cHS4) sequences that diminish promoter interference36,37. b Immunostaining of MC transduced with lentiviral vector shown in (a) and selected with puromycin (ZsGreen+) for the MC marker CD14 (red) prior to differentiation. c HCAEC cells were transduced with the lentiviral dual promoter vector shown in b. They express DsRed but not ZsGreen indicating that CD68 is inactive in HCAEC. d, e Immunostaining for MC marker CD14 and EC markers CD144 and VEGFR2 (red) of puromycin selected MC (ZsGreen+) that were coaxed to differentiate towards EC under (d) static or (e) shear stress conditions. Scale Bar: 20 µm.
