Fig. 4: Optimized Protocol for MC Differentiation.
From: Endothelialization of arterial vascular grafts by circulating monocytes
a Schematic of optimized protocol timeline. b Representative images of Y27 induced spreading of MC on both FN and iVEGFs and subsequent quantification of cell area (µm2) (c) of phalloidin (green) stained cells. +Y27 (gray bars) and –Y27 (white bars); Significance denoted by (****p < 0.0001) compared to surfaces without Y27 using two-way ANOVA with Sidak’s test (DF = 12, F = 81.82); n = 4 independent biological replicates; error bars denote ±SD of the mean. d Representative images of MC stained for the proliferation marker Ki67 (green) after CHIR treatment for 2 days and subsequent quantification of positive cells (e) four asterisk denotes statistical significance (p < 0.0001) compared to No CHIR treatment using one-way ANOVA and Dunnett’s test (DF = 15, F = 42.32); n > 300 cells per n = 6 independent biological replicates; error bars denote ±SD of the mean. f, g Representative brightfield images of MC at the indicated times during the 14-day differentiation on FN (f) or iVEGF (g).
