Fig. 2: Elavl4 isoforms are translationally derepressed in the VZ by mid-neurogenesis. | Nature Communications

Fig. 2: Elavl4 isoforms are translationally derepressed in the VZ by mid-neurogenesis.

From: Translational derepression of Elavl4 isoforms at their alternative 5′ UTRs determines neuronal development

Fig. 2

a Schematic of the four Elavl4 isoforms in mice. All isoforms share a common 3′ UTR sequence (black). Minor differences are in coding regions (exons in gray) and major differences in 5′ UTRs (distinguished by color). NCBI Accession numbers for each variant is indicated underneath common name and the location of qRT-PCR probes used is shown below (red). b Relative mRNA levels of positive control Atxn1 and the different Elavl4 isoforms from FACS-sorted Tbr2-GFP+ and Nestin-GFP+ cells determined by qRT-PCR (n = 3 FACS sorts per age). Data represent the mean and SEM. Data normalized to Gapdh. Statistics: Two-way ANOVA with Tukeyʼs. *p < 0.05. c Fluorescent in situ hybridization of E13 and E16 VZ for Nestin (blue), Atxn1 (green, left), Elavl4-v2 (red, left), Elavl4-v3 (red, right), and Elavl4-v4 (green, right). Scale bar: 20 μm. d, f E13 and E17 neocortices were in utero electroporated (IUE) at E12 (d) or E16 (f), respectively. VZ was transfected with CAG-GFP (green) and either Elavl4-v2-5′ UTR-Renilla, Elavl4-v3-5′ UTR-Renilla or Elavl4-v1&4-5′ UTR-Renilla (red) (n = 3 or 4 animals, respectively). The merged image is on the far right. Arrowheads represent colocalization in VZ. e and g show zoomed-in regions above VZ as indicated. Arrows represent colocalization in cortical plate (CP), intermediate zone (IZ) and subventricular zone (SVZ). DAPI is in blue. d, f scale bar: 40 μm. e, g scale bar: 20 μm.

Back to article page