Fig. 4: Gene expression and metabolic flux analysis of TnikWT and Tnik−/− p14 TEFF cells.
From: TNIK signaling imprints CD8+ T cell memory formation early after priming
a GO enrichment analysis of the biological pathways significantly affected in D6 KO vs WT p14 T cells. b Gene set enrichment analysis (GSEA) of glucose metabolism and oxidative stress. c Gene network and canonical pathway analysis highlighting the regulation and interrelation of metabolic assigned processes. d Heatmap of differentially expressed genes assigned to clusters in KO vs WT effector p14 T cells. e GSEA of the apoptosis pathway and heatmap of differentially expressed genes in the cell death cluster. f GSEA of the PI3K/Akt pathway activation and heatmap of differentially expressed genes in the immune-related signaling cluster. Highlighted genes in the clustered heatmaps are discussed. g, h Extracellular metabolic flux analysis of (g) naive or (h) 3 days activated (H8-DCs) WT and KO p14 T cells. Glycolysis (ECAR) and mitochondrial respiration (OCR) were assessed in response to injections of indicated compounds (top), and parameters of glycolysis and OXPHOS were calculated (bottom). Bas, basal glycolysis and respiration; Cap, glycolytic capacity; Res, glycolytic reserve capacity; Max, maximal respiration; SRC, spare respiratory capacity; ATP, ATP-linked respiration; G, glucose; O, oligomycin; F, FCCP; R/A, rotenone/antimycin A; 2-DG, 2-deoxyglucose. Depicted: WT p14 (black circles), KO p14 (red circles). Data are displayed as means ± SEM. Statistics: g, h two-tailed Student's t test, nonsignificant P > 0.05, *P < 0.05, **P < 0.01. Also see Supplementary Fig. 5 and Supplementary Data 1. Source data are provided as a Source Data file.
