Fig. 6: TNIK signaling activates the Wnt pathway and favors symmetric cell division.

a, b WT (black) and KO (red) p14 T cells were co-cultured with mature bone marrow-derived H8-DCs (H8-DCs) (1:1 ratio). Relative gene expression of indicated genes in FACS-purified p14 T cells is shown 3–96 h post activation. One out of two independent experiments is shown. c CFSE dilution of WT or KO p14 T cells after 72 h of co-culture with mature H8-DCs. Representative data from two independent experiments is shown. d ImageStream X analysis of Numb segregation in mitotic p14 T cells after 48 h of co-culture with H8-DCs; scale bar 10 μm. Analysis includes a total of 375–499 dividing mitotic cells. Representative nuclear stain (DAPI), α-tubulin stain, Numb stain and bright field (BF) depict symmetric division (SD) vs asymmetric division (AD); scale bar 10 μm. e Numb segregation per each culture replicate (n = 2–3) and frequencies of SD vs AD were assessed. Fold change of SD frequency normalized to average of WT control group is depicted. Pooled data from two independent experiments is shown (two replicates per timepoint). f ImageStream X analysis of Numb segregation in mitotic AdTf p14 T cells isolated of spleens 96 h p.i. Frequency of AD vs SD of total dividing WT and KO p14 T cells is shown. Analysis includes a total of 192 (KO) and 186 (WT) dividing mitotic cells from three pooled mice per replicate (n = 4). g Model of cell fate in KO vs WT T cells. Three cell division steps are observed within 48 h post activation. Data are displayed as means ± SEM. Depicted: WT p14 (black lines/circles), KO p14 (red lines/circles). Statistics: a, b, e, f two-tailed Student's t test, nonsignificant P > 0.05, *P < 0.05, **P < 0.01. Also see Supplementary Fig. 6. Source data are provided as a Source Data file.