Fig. 3: Neurodegeneration in the AdarE374A mutant is similar to the Adar5G1 null mutant. | Nature Communications

Fig. 3: Neurodegeneration in the AdarE374A mutant is similar to the Adar5G1 null mutant.

From: Adar RNA editing-dependent and -independent effects are required for brain and innate immune functions in Drosophila

Fig. 3

a–j Neurodegeneration in haematoxylin-eosin stained 5 micron paraffin wax sections of mushroom body in (a) wild-type w1118 day 25, (b) Adar5G1 at day 25 (c) AdarE374A at day 30. Staining of retina in (d) wild-type w1118 day 30 (e) Adar5G1 at day 25 (f) AdarE374A at day 30. Staining of whole brain (g) wild-type w1118 day 30 (h) AdarE374A at day 30. (i) ChAT-GAL4-driven AdarE374A expression in cholinergic neurons is sufficient to prevent neurodegeneration in Adar5G1 mutant mushroom body at day 30 and (j) retina day 30. Scale bars: 20μm. k Adar transcript expression in fly heads is greater in Adar5G1; ChAT > AdarE374A flies expressing Adar from a UAS-AdarE374A construct in cholinergic neurons than it is in wild type w1118 or AdarE374A chromosomal mutant flies. p-value was calculated by Student’s t test *: p-value < 0.05. **: p-value < 0.01. ***: p-value < 0.005: n.s—not significant. Error bars: SEM (Standard Error of Mean for biological replicates).

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