Fig. 6: Adar mutant aberrant AMP expression requires Dcr-2.

a Open field locomotion of Adar mutant and Adar; Dicer2 double mutant flies. p-values were calculated by a one-way ANOVA followed by Tukey’s test. *p-value < 0.05. **p-value < 0.01. ***p-value < 0.005: n.s—not significant. b DIM transcript expression RT-qPCR measurements in Adar mutant and Adar; Dicer-2 double mutant flies. qPCR from 2 day old whole flies. Levels of each AMP transcript in each of the different whole fly RNA samples is compared to that in w¹¹¹⁸. c Rescue of aberrant AMP transcript expression by Dcr-2 RNAi knockdown in Adar^5G1 heads. Levels of each AMP transcript in each of the different fly head RNA samples is compared to that in w¹¹¹⁸. Decrease in the aberrant induction of AMP transcripts in Adar^E374A; ChAT > Dicer2^RNAi and Adar^5G1; ChAT > Dicer2^RNAi heads compared to Adar^E374A and Adar^5G1, respectively, is statistically significant. p value was calculated by Student ‘s t test *p-value < 0.05. **p-value < 0.01. ***p-value < 0.005: n.s—not significant. Error bars: SEM (Standard Error of Mean for biological replicates).