Table 2 IC50s (mean and S.E.M) for GNF179 in the indicated yeast CRISPR-Cas9 edited strains.

From: Pan-active imidazolopiperazine antimalarials target the Plasmodium falciparum intracellular secretory pathway

Strain name

Systematic gene ID

Standard gene name

Mutation

Gene description

GNF179 IC50 (µM)

GNF179-fold resistance (FR)

ABC16-Monster

N/A

N/A

N/A

N/A

47.30 ± 3.8

N/A

EAW361

YBR171W

SEC66

M1I

Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER

70.10 ± 11.7

1.5

EAW277

YBR171W

SEC66

S107*

Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER

74.83 ± 9.4

1.6

EAW289

YCR034W

ELO2

G183C

Fatty acid elongase, involved in sphingolipid biosynthesis;

88.15 ± 8.7

1.9

EAW327

YLR372W

ELO3

Y307*

Fatty acid elongase, involved in sphingolipid biosynthesis;

76.57 ± 10.0

1.6

  1. Strain name, systematic name, 18 h IC50s against GNF179 (mean ± standard error with n ≥ 3) and relative change compared to unedited ABC16-Monster strain are indicated.
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