Table 3 IC50s (mean and S.E.M) for GNF179 in the indicated yeast haploid deletion strains.

From: Pan-active imidazolopiperazine antimalarials target the Plasmodium falciparum intracellular secretory pathway

Strain name

Systematic name

Mutation

Gene description

GNF179 IC50 (µM)

GNF179-fold resistance (FR)

BY4742

N/A

N/A

N/A

121.26 ± 2.9

N/A

sec66Δ

YBR171W

Haploid deletion of sec66

Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER

188.69 ± 11.4

1.6

sec72Δ

YLR292C

Haploid deletion of sec72

Non-essential subunit of Sec63 complex, involved in protein targeting and import into the ER

177.37 ± 5.1

1.5

elo2Δ

YCR034W

Haploid deletion of elo2

Fatty acid elongase, involved in sphingolipid biosynthesis;

254.88 ± 55.1

2.1

sur2Δ

YDR297W

Haploid deletion of sur2

Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine

217.9 ± 12.5

1.8

emp65Δ

YER140W

Haploid deletion of emp65

Integral membrane protein of the ER

152.2 ± 10.5

1.3

atg15Δ

YCR068W

Haploid deletion of atg15

Phospholipase

137.54 ± 12.9

1.1

  1. Strain name, systematic name, 18 h IC50 against GNF179 (mean ± standard error with n ≥ 2) and relative change compared to wild-type are indicated. Strains obtained from yeast deletion collection36.
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