Table 4 Seventy-two-hour SYBR-green IC50s for the indicated compound combinations including GNF179.

From: Pan-active imidazolopiperazine antimalarials target the Plasmodium falciparum intracellular secretory pathway

Plasmodium falciparum drug treatment

GNF179 IC50 (nM)

Fold change in IC50

Artemisinin IC50 (nM)

Chloroquine IC50 (nM)

Dd2

4.31 ± 0.88

–

19.4 ± 1.2

117 ± 14

Dd2 + 1 µM Brefeldin A

0.80 ± 0.22

0.19

21.1 ± 2.7

108.1 ± 17

Dd2 + 5 uM Golgicidin

1.55 ± 0.3

0.36

20.4 ± 1.9

108 ± 18

Dd2 + 250 nM KDU691

1.8 ± 0.35

0.42

18.0 ± 2.4

90.3 ± 6

Dd2 + 1 mM Thapsigargin

4.42 ± 1.4

1.3

18.4 ± 0.8

139 ± 9.8

Dd2 + 1 nM Carmaphycin B

2.6 ± 0.1

0.75

3.5 ± 1.2

90 ± 5.8

Dd2 + 100 nM Cycloheximide

2.6 ± 0.63

0.75

17.5 ± 1.52

101 ± 13.1

  1. Parasites were synchronized to ring stage before IC50s were measured using the SYBR green method. IC50s presented as Mean ± Standard error with ≥2 biological replicates of two technical replicates each.
Back to article page