Fig. 1: Extra-soft oocytes have chromosome alignment defects in metaphase I.
From: Artificially decreasing cortical tension generates aneuploidy in mouse oocytes
a Scheme of the cVCA construct. b Representative time-lapse movies of chromosomes (black, (H2B)-GFP) in control and cVCA oocytes starting 1 h 30 min before anaphase I (10 independent experiments). Scale bar: 10 µm. The red asterisk points at a misaligned chromosome segregated in the oocyte. c Bar graph representing the percentage of oocytes with aligned (black) or not (gray) chromosomes 30 min before anaphase I for controls and cVCA oocytes. n is the number of oocytes analyzed. Data are from ten independent experiments. Statistical significance of differences is assessed with a two-sided χ2-test: **P-value = 0.0003. d Graph representing the aspect ratio (close to 1 is a circle, close to 0 an ellipse as shown in red) for controls and cVCA oocytes. Quantifications 30 min before anaphase I. n is the numbers of oocytes analyzed. Data are from seven independent experiments. Statistical significance of differences between distributions is assessed with a two-sided Kolmogorov–Smirnov test: *P-value = 0.013. e Scheme of the cFH1FH2 construct. f Representative time-lapse movies of chromosomes (black, (H2B)-GFP) in control and cFH1FH2 oocytes starting 1 h 30 min before anaphase I (three independent experiments). Scale bar: 10 µm. The red asterisk points at a misaligned chromosome segregated in the oocyte. g Bar graph representing the percentage of oocytes with aligned (black) or not (gray) chromosomes 30 min before anaphase I for controls and cFH1FH2 oocytes. n is the number of oocytes analyzed. Data are from three independent experiments. Statistical significance of differences is assessed with a two-sided Fisher’s test: *P-value = 0.0201. h Dot plot showing the width of the bounding box containing the chromosomes 30 min before anaphase I in controls and cFH1FH2 oocytes. n is the number of oocytes analyzed. Data obtained from three independent experiments. Black bars and whiskers represent mean and SD. Statistical significance of differences is tested with a two-sided Mann–Whitney test: *P-value = 0.0134. The bounding box corresponds to the yellow square in the image of spindle (green) with chromosomes (blue), its width to the yellow dashed line. Scale bar: 5 µm.
