Fig. 4: Actin organization and density in extra-soft oocytes is similar to controls and its depolymerization does not rescue chromosome alignment.
From: Artificially decreasing cortical tension generates aneuploidy in mouse oocytes
a Cropped images of representative immunofluorescence of control and cVCA oocytes stained for F-actin (gray) at BD + 6 h 30 min. Insets are magnification of the chromosomes (gray) and of regions marked by yellow dashed line boxes. Scale bar: 10 µm. Three independent experiments. b Dot plot representing the F-actin spindle intensity at BD + 6 h 30 min in controls and cVCA oocytes. n is the number of oocytes analyzed. Data are from three independent experiments. Statistical significance of differences is assessed with a two-sided Mann–Whitney test: n.s P-value = 0.6832. c Dot plot representing the F-actin cytoplasm intensity at BD + 6 h 30 min in controls and cVCA oocytes. n is the number of oocytes analyzed. Data are from three independent experiments. Statistical significance of differences is assessed with a two-sided Mann–Whitney test: n.s P-value = 0.8207. d Oocytes at BD + 6 h 30 min expressing Histone(H2B)-GFP (blue) and cVCA (red). The oocyte on the right panel were treated with 1 µg/ml cytochalasin D. Scale bar: 10 µm. Five independent experiments. e Dot plot representing the metaphase I plate width at BD + 6 h 30 min in cVCA oocytes treated or not with 1 µg/ml cytochalasin D. n is the number of oocytes analyzed. Data are from five independent experiments. Statistical significance of differences is assessed with a two-sided Mann–Whitney test: n.s P-value = 0.810. For b, c, e, black bars and whiskers represent mean and SD.
